Germinal Vesicle Stages in Pig Follicular Oocytes Collected by Different Methods
نویسندگان
چکیده
منابع مشابه
Epigenetic reprogramming in somatic cells induced by extract from germinal vesicle stage pig oocytes.
Genomic reprogramming factors in the cytoplasm of germinal vesicle (GV) stage oocytes have been shown to improve the efficiency of producing cloned mouse offspring through the exposure of nuclei to a GV cytoplasmic extract prior to somatic cell nuclear transfer (SCNT) to enucleated oocytes. Here, we developed an extract of GV stage pig oocytes (GVcyto-extract) to investigate epigenetic reprogra...
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During oocyte growth, the morphology of the nucleolus changes into a compact and homogenous structure. The compact nucleoli in full-grown oocytes are not stained by aceto-orcein staining or immunofluorescence staining. In this study, we developed a hematoxylin staining method for pig oocytes in whole-mount preparations to visualize the nucleoli. Nucleoli of growing and full-grown oocytes were s...
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We evaluated the ability of immature pig oocytes (at germinal vesicle stage) to detect differences in the in vitro penetration rates of boar spermatozoa. In Experiment 1, immature and ovulated oocytes (n=303) were exposed to capacitated boar spermatozoa to determine if the penetrability of immature pig oocytes was comparable to that of ovulated oocytes. The percentages of penetrated oocytes and...
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Background The utilization of immature oocytes derived from stimulated cycles could be of great importance, specially for urgent fertility preservation cases. The aim of the current study was to determine whether in-vitro maturation is more successful before or after the vitrification of these oocytes. MaterialsAndMethods The study was performed in a private IVF center. 318 germinal vesicle (GV...
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The regulation of the cell cycle during the transition from prophase to metaphase I was studied by analysing protein changes and introducing protein blocks during the transition phase. The results show that the progression to metaphase in ovine oocytes is totally dependent on new protein synthesis. By delaying the addition of the inhibitor, cycloheximide, for progressively longer periods after ...
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ژورنال
عنوان ژورنال: Journal of Reproduction and Development
سال: 1997
ISSN: 0916-8818,1348-4400
DOI: 10.1262/jrd.43.339